Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8472108 | Immunobiology | 2018 | 7 Pages |
Abstract
We recently found that erythroblast-like cells derived from human leukaemia K562 cells express C5a receptor (C5aR) and produce its antagonistic and agonistic ligand ribosomal protein S19 (RP S19) polymer, which is cross-linked between K122 and Q137 by tissue transglutaminases. RP S19 polymer binds to the reciprocal C5aRs on erythroblast-like cells and macrophage-like cells derived from human monocytic THP-1 cells and promotes differentiation into reticulocyte-like cells through enucleation in vitro. To examine the roles of RP S19 polymer in mouse erythropoiesis, we prepared Q137E mutant RP S19 gene knock-in C57BL/6J mice. In contrast to wild-type mice, erythroblast numbers at the preliminary stage (CD71high/TER119low) in spleen based on transferrin receptor (CD71) and glycophorin A (TER119) values and erythrocyte numbers in orbital artery bloods were not largely changed in knock-in mice. Conversely, erythroblast numbers at the early stage (CD71high/TER119high) were significantly decreased in spleen by knock-in mice. The reduction of early erythroblast numbers in spleen was enhanced by the phenylhydrazine-induced pernicious anemia model knock-in mice and was rescued by a functional analogue of RP S19 dimer S-tagged C5a/RP S19. These data indicated that RP S19 polymer plays the roles in the early erythroblast differentiation of C57BL/6J mouse spleen.
Keywords
FACSPHZDBACD54GPCRsCD11aCFU-EBFU-EC5aRERK1/2G protein-coupled receptorsembryonic stemlymphocyte function antigen-1fluorescence activated cell sortingPhenylhydrazineintracellular adhesion molecule-1Heminerythroid burst-forming unitsDiamond-Blackfan Anemiaextracellular signal-regulated kinase 1/2Glycophorin AC5a receptor
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Authors
Koji Yamanegi, Naoko Yamada, Keiji Nakasho, Hiroshi Nishiura,