| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8475374 | Journal of Molecular and Cellular Cardiology | 2013 | 11 Pages |
Abstract
⺠Human native Ito from atrial myocytes exhibited steep temperature sensitivity. ⺠The influence of β-subunits on Kv4.3 channel kinetics was dependent on temperature. ⺠A Markov state model was developed by incorporating temperature dependency. ⺠The high temperature sensitivity of human Ito could be not mimicked in CHO cells. ⺠Kinetics of native Ito at 37 °C were reproducible with Kv4.3/KChIP2, KCNE2 and DPP6.
Keywords
HEK293β-subunitsKChIP2ΔG0APDFCSQ10GFPPBSAUCChoChinese Hamster OvaryTransient outward currentfoetal calf serumEffective chargeTemperature coefficientPhosphate buffered salineAction potential durationMarkov modelarea under the curveTemperature dependenceaction potentialgreen fluorescent proteinhuman embryonic kidney 293
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Authors
S. Radicke, T. Riedel, D. Cotella, K. Turnow, U. Ravens, M. Schaefer, E. Wettwer,