Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8476390 | Molecular and Cellular Endocrinology | 2018 | 42 Pages |
Abstract
Insulin potentiates leptin effects on muscle accrual and glucose homeostasis. However, the relationship between leptin's central effects on peripheral insulin sensitivity and the associated structural changes remain unclear. We hypothesized that central leptin infusion modifies muscle size through activation of insulin signaling. Muscle insulin signaling, enzymes of fatty acid metabolism, mitochondrial respiratory chain complexes, proliferating cell nuclear antigen (PCNA) and fiber area were analyzed in the gastrocnemius of chronic central infused (L), pair-fed (PF) and control rats. PCNA-positive nuclei, fiber area, GLUT4 and glycogen levels and activation of Akt and mechanistic target of rapamycin were increased in L, with no changes in PF. Acetyl-CoA carboxylase-β mRNA levels and non-esterified fatty acid and triglyceride content were reduced and carnitine palmitoyltransferase-1b expression and mitochondrial complexes augmented in L. These results suggest that leptin promotes an increase in muscle size associated with improved insulin signaling favored by lipid profile.
Keywords
MyogeninGastrocnemiusAMPKPEPCKGLUT4UCP2NEFAIRβMedian fluorescent intensityGSK3β70 kDa ribosomal protein S6 kinase 1p70S6K1PCNARps18RPL13Aribosomal protein L13Aphosphatase and tensin homolog on chromosome 10ribosomal protein S18CREBPI3KMFImTORERKCpt1bcarnitine palmitoyltransferase 1bAMP-activated protein kinaseProliferating Cell Nuclear Antigenfatty acid synthaseNon-esterified fatty acidCSAinterleukinglucose transporter 4Mitochondrial respiratory chainInsulin signalingFasnPhosphatidylinositol 3-kinasephosphoenolpyruvate carboxykinaseLeptincross-sectional areaMYOGMechanistic target of rapamycincyclic AMP response element-binding proteinuncoupling protein 2Ptenextracellular signal-regulated kinaseGlycogen synthase kinase 3β
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Authors
Emma Burgos-Ramos, Sandra Canelles, Amaia RodrÃguez, Laura M. Frago, Javier Gómez-Ambrosi, Julie A. Chowen, Gema Frühbeck, Jesús Argente, Vicente Barrios,