Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8476410 | Molecular and Cellular Endocrinology | 2018 | 30 Pages |
Abstract
We evaluated the impact of high-fat diet (HFD) on ovarian gene expression. Female 5-week-old C57BL/6J mice were fed a 60% HFD or standard chow for 10 weeks. HFD-fed mice were then separated into obese (HF-Ob) and lean (HF-Ln) based on body weight. HFD exposure led to impairment of the estrous cycle, changes in hormones affecting reproduction, and decreased primordial follicles regardless of the development of obesity. RNA-sequencing of whole ovaries identified multiple genes with altered expression after HFD, with 25 genes displaying decreased expression in both HF-Ln and HF-Ob mice compared to the chow-fed controls (q < 0.05). Several of these 25 genes are involved in normal ovarian functions, including ovulation (Edn2, Tnfaip6, Errfi1, Prkg2, and Nfil3), luteinization (Edn2), and luteolysis (Nr4a1). Taken together, elevated dietary fat intake, regardless of obesity, is associated with impaired estrous cycle, depletion of the ovarian reserve, and altered expression of genes critical to normal ovulatory function.
Keywords
ADAMTS1qRT-PCRLEPSOCS3HFDNHPPBtg2TSHTNFAIP6ATF3DIONfil3ERRFI1NR4A1AMHfemale fertilityOvaryRNA sequencingstandard error of the meanhigh-fat dietsuppressor of cytokine signaling 3transcription factor 3FosBLeptinDifferentially expressedSEMObesityfollicle-stimulating hormoneluteinizing hormonethyroid stimulating hormoneFSHquantitative reverse transcription polymerase chain reactiondiet-induced obesity
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cell Biology
Authors
Natalie M. Hohos, Kirstin J. Cho, Delaney C. Swindle, Malgorzata E. Skaznik-Wikiel,