Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8476723 | Molecular and Cellular Endocrinology | 2016 | 28 Pages |
Abstract
It has been implicated that IGF-1 secretion can be regulated by dietary protein. However, whether the dipeptides, one of digested products of dietary protein, have influence on IGF-1 secretion remain largely unknown. Our study aimed to investigate the effects of the dipeptide Pro-Asp on IGF-1 secretion and expression in hepatocytes and to explore the possible underlying mechanisms. Our findings demonstrated that Pro-Asp promoted the secretion and gene expression of IGF-1 in HepG2 cells and primary porcine hepatocytes. Meanwhile, Pro-Asp activated the ERK and Akt signaling pathways, downstream of IGF-1. In addition, Pro-Asp enhanced GH-mediated JAK2/STAT5 signaling pathway, while inhibition of JAK2/STAT5 blocked the promotive effect of Pro-Asp on IGF-1 secretion and expression. Moreover, acute injection of Pro-Asp stimulated IGF-1 expression and activated JAK2/STAT5 signaling pathway in mice liver. Together, these results suggested that the dipeptide Pro-Asp promoted IGF-1 secretion and expression in hepatocytes by enhancing GH-mediated JAK2/STAT5 signaling pathway.
Keywords
IGF-1PEPT2STAT5Janus Kinase 2RIAJAK2ERKASPPI3KHepatocytesAspartic acidAktdipeptideradioimmunoassayinsulin-like growth factor 1signal transducer and activator of transcription 5Phosphatidylinositol 3-kinasePROGrowth hormonepeptide transporter 1protein kinase BProlinePept1extracellular signal-regulated kinase
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Authors
Songbo Wang, Guoqing Wang, Mengyuan Zhang, Lu Zhuang, Xiaojuan Wan, Jingren Xu, Lina Wang, Xiaotong Zhu, Ping Gao, Qianyun Xi, Yongliang Zhang, Gang Shu, Qingyan Jiang,