Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8476831 | Molecular and Cellular Endocrinology | 2016 | 31 Pages |
Abstract
Cholinergic anti-inflammatory pathway (CAP) prevents inflammatory cytokines production. The main was to evaluate the effect of maternal obesity on cholinergic pathway in the offspring. Female mice were subjected to either standard chow (SC) or high-fat diet (HFD) during pregnancy and the lactation period. After weaning, only male offspring from HFD dams (HFD-O) and from SC dams (SC-O) were fed the SC diet. Key proteins of the CAP were downregulated and serum TNF-α was elevated in the HFD-O mice. STAT3 and NF-κB activation in HFD-O mice ICV injected with nicotine (agonist) were lower than SC-O mice. Basal cholinesterase activity was upregulated in HFD-O mice in both investigated tissues. Lipopolysaccharide increased TNF-α and IL-1β expression in the liver and WAT of SC-O mice, but this effect was greater in HFD-O mice. In conclusion these changes exacerbated cytokine production in response to LPS and contributed to the reduced sensitivity of the CAP.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cell Biology
Authors
Tanyara Baliani Payolla, Simone Ferreira Lemes, ThaÃs de Fante, Andressa Reginato, Cristiano Mendes da Silva, Thayana de Oliveira Micheletti, Hosana Gomes Rodrigues, Adriana Souza Torsoni, Marciane Milanski, Marcio Alberto Torsoni,