Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8477198 | Molecular and Cellular Endocrinology | 2014 | 11 Pages |
Abstract
8-Prenylnaringenin (8-PN) and naringenin (Nar) are phytoestrogens found in food items and nutritional supplements, while 6-(1.1-dimethylallyl)naringenin (6-DMAN) is a component of an African plant. Besides their assumed beneficial effects they may promote mammary and endometrial cancer. We therefore assessed their proliferative and estrogenic potential on the mammary gland in vitro and in vivo. In competitive estrogen receptor (ER) ligand binding assays 8-PN displayed a high relative binding affinity for both ERs with a preference for ERα and had the strongest mitotic effect on MCF-7 cells among the test substances. In a three day exposure in young adult ovariectomized female rats 15 mg/kg 8-PN had the highest capacity to increase the number of terminal end buds (TEB) in the mammary gland and stimulated expression of proliferation markers in epithelial ductal cells, followed by 6-DMAN and Nar, but overall their capacity to stimulate proliferation was weak in comparison to 17β-Estradiol (E2).
Keywords
Fulvestrantribosomal protein S18TGF-βBERKORps18PRKOPCNATEBCEEMKi67CSN28-PN8-prenylnaringeninFULEGFRAREGEGFovariectomizedOVXIGF-1NARHRTRBAPGRFGF17β-estradiolamphiregulinProliferating Cell Nuclear AntigenConjugated equine estrogenTransforming growth factor βUterotrophic assayMCF-7 cellsepidermal growth factorInsulin-like growth factorNaringeninhormone replacement therapyRelative binding affinityTerminal end budEstrogen receptorEpidermal growth factor receptorProgesterone receptor
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Authors
Janina Helle, Kristin Kräker, Manuela I. Bader, Annekathrin M. Keiler, Oliver Zierau, Günter Vollmer, JoEllen Welsh, Georg Kretzschmar,