Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8477318 | Molecular and Cellular Endocrinology | 2013 | 11 Pages |
Abstract
Mitochondria are essential sites for steroid hormone biosynthesis. Mitochondria in the steroidogenic cells of the adrenal, gonad, placenta and brain contain the cholesterol side-chain cleavage enzyme, P450scc, and its two electron-transfer partners, ferredoxin reductase and ferredoxin. This enzyme system converts cholesterol to pregnenolone and determines net steroidogenic capacity, so that it serves as the chronic regulator of steroidogenesis. Several other steroidogenic enzymes, including 3β-hydroxysteroid dehydrogenase, 11β-hydroxylase and aldosterone synthase also reside in mitochondria. Similarly, the mitochondria of renal tubular cells contain two key enzymes participating in the activation and degradation of vitamin D. The access of cholesterol to the mitochondria is regulated by the steroidogenic acute regulatory protein, StAR, serving as the acute regulator of steroidogenesis. StAR action requires a complex multi-component molecular machine on the outer mitochondrial membrane (OMM). Components of this machine include the 18 kDa translocator protein (TSPO), the voltage-dependent anion chanel (VDAC-1), TSPO-associated protein 7 (PAP7, ACBD3), and protein kinase A regulatory subunit 1α (PKAR1A). The precise fashion in which these proteins interact and move cholesterol from the OMM to P450scc, and the means by which cholesterol is loaded into the OMM, remain unclear. Human deficiency diseases have been described for StAR and for all the mitochondrial steroidogenic enzymes, but not for the electron transfer proteins or for the components of the cholesterol import machine.
Keywords
OMMSREBPsP450sccscavenger receptor B1LALpTHHSLTSPOSR-B1NPCCRAC3β-Hydroxysteroid dehydrogenaseHMGCoAVDAC13βHSDNiemann Pick type CIMSflavin adenine dinucleotidePAP7HDLACTHNADPHPBRSterol regulatory element binding proteinsPCPMLN64ANTACATpKaSF11,25(OH)2Dacyl transferaseSteroidogenesisCholesterol transportimmFADadenine nucleotide transporterinner mitochondrial membraneMentalSoatStarendoplasmic reticulumstartSteroidogenic factor 1outer mitochondrial membranelysosomal acid lipaseLDLlow-density lipoproteinsMichaelis constantnicotinamide adenine dinucleotide phosphateadrenocorticotropic hormoneluteinizing hormoneparathyroid hormoneVitamin DSteroidogenic acute regulatory proteinprotein kinase Avoltage-dependent anion channelperipheral benzodiazepine receptor
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Authors
Walter L. Miller,