Protein nitration promotes inducible nitric oxide synthase transcription mediated by NF-κB in high glucose-stimulated human lens epithelial cells

► High glucose could cause nitration of nuclear NF-κB p65 and its co-activator p300. ► Protein nitration promoted the interaction between NF-κB and p300. ► Protein nitration promoted the NF-κB binding to the site −5212 in iNOS promoter.