| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8477591 | Molecular and Cellular Endocrinology | 2013 | 13 Pages |
Abstract
⺠Single-cell quantitative PCR of channel transcripts in GnRH neurons was validated and used. ⺠E2 increased or decreased the mRNA expression of ion channels in GnRH neurons. ⺠Altered channel mRNA expression leads to changes in the excitability of GnRH neurons.
Keywords
qPCRHCNPOAcanonical transient receptor potentialGnRHTRPCHyperpolarization-activated cyclic nucleotide-gatedGPR5417β-estradiolSingle cell PCRmRNA quantificationafterhyperpolarizationreverse transcriptionZeitgeber timeAHPPreoptic areaLH surgeGnRH neuronsGonadotropin-releasing hormoneluteinizing hormonequantitative real-time PCRhigh voltage activatedHVA
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Authors
Martha A. Bosch, Karen J. Tonsfeldt, Oline K. Rønnekleiv,