| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8477742 | Molecular and Cellular Endocrinology | 2011 | 15 Pages |
Abstract
⺠The molecular basis for tissue and cell type-specific expression of GHSR has not yet been fully elucidated. ⺠The 5â²-flanking region of the rat Ghsr gene was structurally and functionally characterized. ⺠The minimal promoter region was mapped to a region 200-bp upstream from the novel 5â²-untranslated first exon. ⺠DNA methylation and histone modifications at the Ghsr proximal promoter were highly correlated with mRNA expressions.
Keywords
UCSCGHSRinositol triphosphateRLMIP1DnmtGPCRIP3PMSFqRT-PCRHDACMSPinositol monophosphateGHSTSATSS5-Aza-dCFBSDMEM5-aza-2-deoxycytidineDNA methyltransferaseERK1/2MAPKMethylation-specific PCRESTchromatin immunoprecipitationGene expressionUntranslatedGrowth hormone secretagogueTrichostatin Arapid amplification of cDNA endsExpressed Sequence TagCpG islandUniversity of California, Santa Cruztranscription start sitefetal bovine serumtransmembranephenylmethylsulfonyl fluoridePromoter methylationDulbecco’s modified eagle’s mediumRaceGrowth hormonehistone deacetylasequantitative real-time PCRmitogen activated protein kinaseCHiPextracellular signal-regulated kinase 1/2growth hormone secretagogue receptorGhrelin receptorG protein-coupled receptor
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Authors
Hiroshi Inoue, Yukiko Sakamoto, Natsumi Kangawa, Chizuko Kimura, Tsutomu Ogata, Kenji Fujieda, Zhi Rong Qian, Toshiaki Sano, Mitsuo Itakura,