| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8477827 | Molecular and Cellular Endocrinology | 2011 | 6 Pages |
Abstract
⺠We determined whether AMPK affects Wnt/β catenin signaling in osteoblast-like Saos-2 cells. ⺠AICAR and metformin decreased Wnt3a-induced TCF-dependent transcriptional activity. ⺠Metformin suppressed LiCl-induced Wnt transactivation. ⺠Metformin elevated the phosphorylation of β-catenin leading to β-catenin degradation. ⺠In Saos-2 cells, AMPK attenuates Wnt/β catenin signaling by reducing β-catenin protein levels.
Keywords
GAPDHAMPKAPCTCFLiClGSK3βCK1WntTBSLRPAXINPVDFJnkAICARTGF-β5-Aminoimidazole-4-carboxamide ribosideAMP-activated protein kinaseSDS–PAGEadenomatous polyposis coliAraAOsteoblastsodium dodecyl sulfate–polyacrylamide gel electrophoresisβ-catenintransforming growth factor-betaTris-buffered salinepolyvinylidene difluorideconditioned medialithium chlorideMetforminBMPLow-density lipoprotein receptor-related proteinbone morphogenic proteincasein kinase 1glyceraldehyde-3-phosphate dehydrogenase
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Authors
Tomozumi Takatani, Masanori Minagawa, Rieko Takatani, Kaori Kinoshita, Yoichi Kohno,