Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8478336 | Molecular and Cellular Neuroscience | 2018 | 58 Pages |
Abstract
Olfactory ensheathing cells (OECs) migrate from olfactory epithelium towards olfactory bulb (OB), contributing to formation of the presumptive olfactory nerve layer during development. However, it remains unclear that molecular mechanism of regulation of OEC migration in OB. In the present study, we found that OECs highly expressed the receptors of semaphorin 3A (Sema3A) in vitro and in vivo, whereas Sema3A displayed a gradient expression pattern with higher in inner layer of OB and lower in outer layer of OB. Furthermore, the collapse assays, Boyden chamber migration assays and single-cell migration assays showed that Sema3A induced the collapse of leading front of OECs and inhibited OEC migration. Thirdly, the leading front of OECs exhibited adaptation in a protein synthesis-independent manner, and endocytosis-dependent manner during Sema3A-induced OEC migration. Finally, Sema3A-induced collapse of leading front was required the decrease of focal adhesion and a retrograde F-actin flow in a cofilin activation-dependent manner. Taken together, these results demonstrate that Sema3A as an inhibitive migratory factor for OEC migration through cofilin activation is involved in the formation of olfactory nerve layer.
Keywords
Neuropilin-2GDNFNP1PFAMCLRT-PCREPLONLCHXMCDPAOSema3AOECsHGFFBSBDNFanisomycinSprague-Dawleyolfactory epitheliumPhenylarsine oxideAdaptationfetal bovine serumOlfactory Ensheathing CellsSemaphorin 3AcycloheximideHepatocyte growth factorbrain derived neurotrophic factorGlial cell line-derived neurotrophic factorLamina propriaexternal plexiform layermitral cell layerolfactory nerve layerglomerular layermonodansyl cadaverineBMPreverse transcription-PCRMigrationneuropilin-1paraformaldehydeBone morphogenetic proteinolfactory bulbCofilin
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Authors
Ying Wang, Xiaomei Bao, Shiyang Wu, Xiya Shen, Fan Zhang, Zhaoting Lv, Qian Wu, Changnan Xie, Huitao Liu, Jian Lin, Honglin Teng, Zhihui Huang,