Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8486828 | Vaccine | 2011 | 10 Pages |
Abstract
Human enterovirus 71 (HEV71) has emerged as a major cause of epidemics of hand, foot and mouth disease associated with severe neurological sequelae in the Asia-Pacific region. In this study, a passive protection mouse model was used to evaluate the protective efficacy of formalin-inactivated HEV71 vaccines derived from a Chinese C4 genotype strain. Pregnant mice were immunised using a prime/boost strategy and â¥50 U of vaccine protected five-day-old pups from lethal challenge with a mouse-adapted (B3 genotype) strain of HEV71. Immunised mice developed a neutralising antibody response to both the immunising C4 strain and to the mouse-adapted strain. Mice born to immunised dams showed significantly less myositis and reduced viral loads in tissues.
Keywords
DMEMCPEODSTCID50HFMDi.p.HEV71VLP50% tissue culture infectious doseDulbecco's modified Eagle MediumCytopathic effectHuman enterovirus 71optical densitiesELISAEnzyme-linked immunosorbent assayCross-protectionintraperitonealhand, foot and mouth diseaseCNSVirus-like-particlesRhabdomyosarcomaembryonic daycentral nervous systemInactivated vaccine
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Authors
Emily Jane Bek, Khairunnisa Mohamed Hussain, Patchara Phuektes, Chee Choy Kok, Qiang Gao, Fang Cai, Zhenglun Gao, Peter Charles McMinn,