Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8497632 | Developmental & Comparative Immunology | 2018 | 38 Pages |
Abstract
Here we evaluated neonatal transcription of α, β, γ and δ TCR and the γδ T cell co-receptor family WC1 in peripheral blood mononuclear cells. A previous report showed a rapid and global shift in transcription of immunoglobulin genes in neonatal calves during the first month after birth but this was not found here for the T cell genes. Transcription frequency of genes within TRAV subgroups correlated with the number of members, indicating a stochastic choice. In contrast, of the approximately 60 TRDV genes those in two of eleven TRDV1 clades and TRDVb3 were transcribed significantly more than the others while those in only one TRBV subgroup were. Transcription of genes in the TRGV5-containing cassette predominated among TRGV genes as a result of their exclusive usage by the WC1+ γδ T cells with a preference for transcription of two of four TRGV genes in that cassette. Finally, we report no large differences in transcription frequencies among the 13 WC1 genes.
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Authors
Payal Damani-Yokota, Alexandria Gillespie, Yfke Pasman, Daniel Merico, Timothy K. Connelley, Azad Kaushik, Cynthia L. Baldwin,