Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8497995 | Developmental & Comparative Immunology | 2016 | 11 Pages |
Abstract
Immunoglobulin light chain (IgL) is necessary for the assembly of an Ig molecule, which plays important roles in the immune response. IgL genes were identified in various teleost species, but the basic functions of different IgL isotypes and the preferential combination between IgL and IgH (Ig heavy chain) isotypes remain unclear. In the current study, by EST database searching and cDNA cloning in rainbow trout, 8 IgL sequences were obtained, which could be classified into the IgLκF, IgLκG, IgLÏ and IgLλ isotypes, respectively. Trout IgL isotypes were highly expressed in the immune-related tissues, and participated in the immune responses in spleen and gut by stimulation with LPS and poly (I:C). The results of FACS and LC-MS/MS indicated that the IgLκG and IgLÏ isotypes preferentially bonded with the heavy chains of IgM and IgT, respectively, in trout B cells and serum. In addition, the genomic organization of trout IgL isotypes and the utilization of recombination signal sequences were studied.
Keywords
IgHHKLjoining segmentJ segmentV domainIgMC domainRAGPBLRSSFACSORFBCRIGTFBSqPCRimmunoglobulin IGLrecombination signal sequenceConstant domainVariable domainlight chain variable domainheavy chain variable domainfluorescence-activated cell sortingDouble negativeimmunoglobulin light chainheavy chainImmunoglobulin heavy chainfetal bovine serumMass spectrometryOpen reading framesRainbow troutPeripheral blood leukocytequantitative real-time PCRAntibodyliquid chromatographyB cell receptor
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Authors
Nu Zhang, Xu-Jie Zhang, Yu-Long Song, Xiao-Bing Lu, Dan-Dan Chen, Xiao-Qin Xia, J. Oriol Sunyer, Yong-An Zhang,