Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8500324 | International Journal for Parasitology | 2008 | 7 Pages |
Abstract
The aim of our study was to establish a new PCR protocol for the detection and discrimination of Echinococcus granulosus complex on one hand and Echinococcus multilocularis in formalin-fixed, paraffin-embedded tissues (FFPTs) on the other. The target sequences for all PCRs are located on a 471Â bp segment of the mitochondrial ND1 gene, the fragment sizes of the amplification products are 295Â bp (for the sheep strain of E. granulosus), 204Â bp (for the pig strain of E. granulosus) and 252Â bp (for E. multilocularis), respectively. In total, 80 FFPTs from patients with histologically confirmed echinococcosis (76 with E. granulosus and four with E. multilocularis) operated on in Austrian hospitals between 1978 and 2005 were examined. In 68 (85%) samples, we were able to detect specific DNA fragments with our newly established PCR protocols. Thirty-eight (47.5%) of 80 clinical samples were identified as the G1 strain, 26 (32.5%) as the G5, 6 or 7 strains and four (5%) as E. multilocularis. The specificity of all three PCRs was 100%; for the discrimination between G6 and G7 strains, sequencing of an additional 234Â bp PCR fragment was necessary and showed that three out of 26 G5, 6 or 7 PCR-positive patients were infected with E. granulosus genotype G6 (the camel strain).
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Authors
Renate Schneider, Bernd Gollackner, Bernhard Edel, Katharina Schmid, Friedrich Wrba, Georg Tucek, Julia Walochnik, Herbert Auer,