Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8526296 | Biomedicine & Pharmacotherapy | 2018 | 7 Pages |
Abstract
Hepatitis B virus (HBV) is associated with the development of a wide spectrum of liver diseases. The involvement of miRNAs in HBV replication is being gradually identified. Among these miRNAs, miR-146a expression was found to be positively correlated with HBV replication levels. However, the regulatory relationship between miR-146a and HBV replication is still unclear. In the present study, miR-146a was upregulated in HBV-expressing HepG2.2.15 cells compared with HepG2 cells. Overexpression of miR-146a or knockdown of Zinc finger E-box-binding homeobox 2 (ZEB2) promoted HBV replication and expression, while downregulation of miR-146a or overexpression of ZEB2 suppressed HBV replication and expression. In addition, miR-146a was demonstrated to directly target ZEB2. Furthermore, ZEB2 silencing abated anti-miR-146a-induced inhibition on HBV replication and expression. These findings suggested that miR-146a promoted HBV replication by targeting ZEB2, providing a new antiviral strategy for HBV infection.
Keywords
NFIAIFN-αDMEMFBSCHBmiRNAsCFHZEB2HBsAgHBeAgHCCNucleotide analoguesInterferon-αmiR-146aELISAEnzyme-linked immunosorbent assayComplement factor HmicroRNAsfetal bovine serumVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)Dulbecco’s modified eagle’s mediumchronic hepatitis BHBVhepatitis B virusHepatocellular carcinoma
Related Topics
Health Sciences
Medicine and Dentistry
Oncology
Authors
Yanjing Wang, Yuanyuan Li,