Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8527743 | Chinese Journal of Natural Medicines | 2016 | 7 Pages |
Abstract
Tanreqing injection (TRQ), a well-known traditional Chinese medicine formula, is commonly used to treat respiratory diseases. In the present study, a rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determinate the plasma contents of 5 major constituents of TRQ, including chlorogenic acid (CHA), caffeic acid (CFA), baicalin (BA), ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA) in rats after intravenous administration of TRQ. Chromatographic separation was performed on an Agilent Zorbax SB-C18 column (3.5 μm, 100 mm à 2.1 mm), with acetonitrile and 0.1% aqueous formic acid as mobile phase at a flow rate of 0.3 mL·minâ1. The calibration curves were linear over the ranges of 27.0-13 333.0 ng·mLâ1 for CFA, 30.0-14 933.0 ng·mLâ1 for CHA, 50.0-50 333.0 ng·mLâ1 for BA, 550.0-55 000.0 ng·mLâ1 for UDCA, and 480.0-48 000.0 ng·mLâ1 for CDCA, respectively. Intra- and inter-day precisions (relative standard deviations, RSDs) were from 3.11% to 14.08%. The extraction recoveries were greater than 71% and accuracy (relative recovery) was from 89% to 137% for all analytes, except endogenous bile acids. This validated method was successfully applied to the first pharmacokinetic study of CFA, CHA, BA, UDCA and CDCA in rat plasma after intravenous administration of TRQ.
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Authors
Feng ZHANG, Liang SUN, Shou-Hong GAO, Wan-Sheng CHEN, Yi-Feng CHAI,