Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8528939 | European Journal of Pharmacology | 2018 | 40 Pages |
Abstract
We synthesized and investigated the effects of a novel peptide B, RRFSLLRY as a novel PAR-2 antagonist. Peptide B decreased calcium levels in HaCat cells stimulated with trypsin and PAR-2 activator (SLIGKV) and cytokeratin 14 and PCNA was decreased by peptide B. Peptides B also reduced the expression of inflammatory cytokines such as TNF-α and interleukin-6. Significant increase of oxazolone-induced transepidermal water loss (TEWL) was decreased by the addition of peptide B in hairless mice. These findings suggest that the anti-inflammatory effect of peptide B is due to inhibition of PAR-2 signaling. Skin safety of peptide B was demonstrated by performing MTT assay and human repeated insult patch test. Our findings indicate that peptide B might have potential as an anti-inflammatory agent without irritating skin for use in cosmetics and medical treatment of dermatologic disorders.
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Authors
Bora Kim, Hyun-Soo Kim,