Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8530099 | European Journal of Pharmacology | 2016 | 26 Pages |
Abstract
Hypoxia inducible factor (HIF)-1α, a subunit of HIF transcription factor, regulates cellular response to hypoxia. In normoxic conditions, it is hydroxylated by prolyl hydroxylase (PHD)-2 and targeted for proteosomal degradation. Drugs which inhibit PHD-2 have implications in conditions arising from insufficient blood supply. β-ODAP (β-N- oxalyl-L-α, β- diaminopropionic acid), a non-protein excitatory amino acid present in Lathyrus sativus, is an α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor agonist known to activate conventional protein kinase C and stabilize HIF-1α under normoxic conditions. However, the mechanism of HIF-1α stabilization by this compound is unknown. In silico approach was used to understand the mechanism of stabilization of HIF-1α which revealed β-ODAP interacts with key amino acid residues and Fe2+ at the catalytic site of PHD-2. These results were further corroborated with luciferase HRE (hypoxia response element) reporter system in HeLa cells. Different chemical modulators of PHD-2 activity and HIF-1α levels were included in the study for comparison. Results obtained indicate that β-ODAP inhibits PHD-2 and facilitates HIF dependent HRE expression and hence, might be helpful in conditions arising from hypoxia.
Keywords
PHDβ-N-oxalyl-L-α,β-diaminopropionic acidβ-ODAPN-acetyl l-cysteineODDDOxygen dependent degradation domainNanomolar concentrationxCTdimethyloxalylglycineDMOGSDSHREHIFRMSDDTTvdWAdenosine TriphosphateATPEDTAdithiothreitolsodium dodecyl sulfatehypoxia response elementshypoxia response elementHypoxia-inducible factorroot mean square deviationnicotinamide adenine dinucleotide dehydrogenase
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Authors
Ravi Kumar Eslavath, Deepshikha Sharma, Nabil A.M. Bin Omar, Rajasekhar Chikati, Mahesh Kumar Teli, G.K. Rajanikant, Surya S. Singh,