Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8530932 | International Immunopharmacology | 2018 | 6 Pages |
Abstract
Klebsiella pneumoniae (K. pneumoniae) is a hospital-acquired infectious agent that causes a range of diseases. Herein we have developed a novel CXCL8-IP10 hybrid protein and evaluated its efficacy in an animal model of K. pneumoniae infection. Neutrophil chemotaxis data revealed that CXCL8-IP10 could inhibit human neutrophil chemotactic responses induced by the ELR-CXC chemokine CXCL8. To evaluate the effect of CXCL8-IP10 on K. pneumoniae infection, C57BL/6 mice were challenged with K. pneumoniae followed by treatment with CXCL8-IP10 (500â¯Î¼g/kg, i.p.), or dexamethasone (0.8â¯mg/kg, s.c.), or ceftazidime (200â¯mg/kg, s.c.) individually. CXCL8-IP10, dexamethasone or ceftazidime markedly inhibit Klebsiella-induced pulmonary inflammation as assessed by gross examination and histopathology. Moreover, the chemotactic responses of neutrophils was blocked by CXCL8-IP10 rather than dexamethasone or ceftazidime. Furthermore, the levels of inflammatory factors IL-1β, IFN-γ and TNF-α were decreased after CXCL8-IP10, dexamethasone or ceftazidime treatment. Together, these results suggest that CXCL8-IP10 may provide a novel strategy for treating K. pneumoniae infection.
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Authors
Zhangbo Chen, Xiangyu Chen, Hsi-Tsung Cheng, Shu-Chi Yeh, Hui-Yuan Yu, Jya-Wei Cheng, Fang Li,