Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8534443 | Life Sciences | 2018 | 24 Pages |
Abstract
Alcohol, a psychoactive drug is one of the lifestyle factors responsible for male infertility. Present study was carried out to investigate the ameliorative effect of pâcoumaric acid (PCA), a plant derived bioactive phenolic compound on alcohol induced chronic reproductive toxicity in male rats. Thirty male Wistar rats were divided into five groups, each with six animals. Group I as control received vehicle (distilled water) alone. And the following Group II, III, IV, V were treated orally with sequentially (per week) increased dose of ethanol 25% v/v (5, 8, 10 and 12â¯g/kg b wt per week in each group) for 28â¯days. On the 3rd and 4th week, the groups III, IV, V were administered with pâcoumaric acid orally at three different concentrations (Low 50â¯mg/kg b wt; Medium 100â¯mg/kg b wt; High 200â¯mg/kg b wt). The rats treated with ethanol showed abnormal sperm characteristics, reduced anti-oxidant level, reduced testosterone level and abnormal testicular histoarchitecture while the rats treated with PCA in addition to ethanol were found to have protective effects on sperm parameters and apoptosis. The increased caspase-3, caspase-7, p21 immunoreactivity and reduced Cdk4 immunoreactivity in ethanol treated rats confirmed that ethanol increases the apoptosis in testis and a reduced expression in the rats treated with PCA in addition to ethanol indicates a protective role of PCA. Overall, our results showed that PCA mitigates alcohol induced male reproductive toxicity and improves reproductive health in male Wistar rats.
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Authors
Kumari Nishi, Sabitha Ramakrishnan, Vinoth Prasanna Gunasekaran, Kaushal Parkash, Arumugam Ramakrishnan, Natesan Vijayakumar, Mathan Ganeshan,