Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8534575 | Life Sciences | 2018 | 8 Pages |
Abstract
Diabetic cardiomyopathy (DC) describes diabetes-associated changes in the structure and function of myocardium that are not directly linked to other factors such as hypertension. Currently there are some models of DC; however, they take a large time period to mimic key features. In the present study, we investigated the effects of a short-term high-fat/high salt diet (HFHS) treatment on myocardial function and structure, and vascular reactivity in C57BL/6 male mice. After 14â¯weeks HFHS induced hypertension (MAPâ¯=â¯144.95â¯Â±â¯16.13 vs 92.90â¯Â±â¯18.95â¯mmâ¯Hg), low glucose tolerance (AUCâ¯=â¯1049.01â¯Â±â¯74.79 vs 710.50â¯Â±â¯52.57â¯a.u.), decreased insulin sensitivity (AUCâ¯=â¯429.83â¯Â±â¯35.22 vs 313.67â¯Â±â¯19.55â¯a.u.) and increased adiposity (epididymal fat weight 0.96â¯Â±â¯0.10 vs 0.59â¯Â±â¯0.06 OW/BWâ¯Ãâ¯102), aspects present in metabolic syndrome. Cardiac evaluation showed diastolic dysfunction (E/A ratioâ¯=â¯1.20 vs 1.90â¯u.a.) and cardiomyocyte hypertrophy (cardiomyocyte areaâ¯=â¯502.82â¯Â±â¯31.46 vs 385.58â¯Â±â¯22.11â¯Î¼m2). Lastly, vascular reactivity was impaired with higher contractile response (136.10â¯Â±â¯3.49 vs 120.37â¯Â±â¯5.43%) and lower response to endothelium-dependent vasorelaxation (74.01â¯Â±â¯4.35 vs 104.84â¯Â±â¯3.57%). In addition, the diet was able to induce an inward coronary remodeling (vascular total area: SCNS 6185â¯Â±â¯800.6 vs HFHS 4085â¯Â±â¯213.7â¯Î¼m2). Therefore, we conclude that HFHS short-term treatment was able to induce metabolic syndrome-like state, cardiomyopathy and vascular injury working as an important tool to study cardiometabolic diseases.
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Authors
Leônidas Graças Mendes-Junior, Leandro Ceotto Freitas-Lima, JanaÃna Ribeiro Oliveira, Marcos B. Melo, Jonh David Feltenberger, Igor Viana Brandi, Bruna Mara Aparecida Carvalho, André Luiz Sena Guimarães, Alfredo MaurÃcio Batista De Paula,