CXCL1 activates TRPV1 via Gi/o protein and actin filaments

The putative signaling pathway between CXCR2 receptors and TRPV1 upon acute application of CXCL1. After the ligand binding, the CXCR2 receptor couples to Gi/o protein and activates protein kinase pathways that can induce diverse intracellular effects, including phosphorylation of CXCR2. After activation, the receptor is internalized through a clathrin-mediated phosphorylation-dependent pathway and interacts with the actin filaments trough β-arrestin or another protein. Changes in the actin filaments are then transmitted to TRPV1 receptors which respond in a slow, low amplitude manner, allowing an influx of Na+ and Ca2 + ions. Questions marks indicate unknown molecules/mechanisms that mediate the interactions between actin filaments and CXCR2 or TRPV1.123