Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8537162 | Progress in Neuro-Psychopharmacology and Biological Psychiatry | 2019 | 42 Pages |
Abstract
Bath perfusion of ketamine, but not the NMDA receptor antagonist D-AP5, increased the frequency of AMPA receptor-mediated spontaneous EPSCs (sEPSCs) in DR neurons. However, ketamine did not affect evoked EPSCs or spontaneous inhibitory currents (sIPSCs). Pre-incubation of DR slices with the mTOR inhibitor PP242 decreased the frequency of sEPSCs and prevented the effect of ketamine. The results also show that while no electrophysiological effects were detected 24â¯h after ketamine administration, phosphorylation levels of mTOR were significantly increased in the DR. Nevertheless, expression levels of synaptic proteins were unaffected at that time. Altogether, the present data demonstrate that ketamine transiently increases spontaneous AMPA receptor-mediated neurotransmission in the DR.
Keywords
PFCmTORaCSFPSD-95MK-801eEPSCsTSTDNQXsEPSCssIPSCsp-mTORd-AP5DMSOspontaneous excitatory postsynaptic currentsSpontaneous inhibitory postsynaptic currentsDimethyl sulfoxideprefrontal cortexartificial cerebrospinal fluidevoked excitatory postsynaptic currentsmammalian target of rapamycindorsal raphe nucleuspostsynaptic density protein 95
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Authors
Nerea Llamosas, Laura Perez-Caballero, Esther Berrocoso, Cristina Bruzos-Cidon, Luisa Ugedo, Maria Torrecilla,