Glyphosate affects the secretion of regulators of uterine contractions in cows while it does not directly impair the motoric function of myometrium in vitro

The effects of pure glyphosate and its most popular product (brand name: Roundup) on the secretion of hormones involved in the regulation of myometrial contractions as well as their direct effects on myometrial contractions were examined. Myometrial strips as well as uterine and ovarian cells were taken from cows during the oestrous cycle and they were treated with both compounds at concentrations from their environmental range. Glyphosate stimulated the secretion of oestradiol from granulosa cells while both herbicides increased and decreased oxytocin (OT) and progesterone secretion from luteal cells respectively. However only Roundup stimulated mRNA expression of the precursor of OT. Both compounds decreased the secretion of prostaglandins from endometrial cells while they exerted no effect on the basal and OT-stimulated force of myometrial contractions. The studied herbicides did not directly impair the motoric function of the myometrium. However our data indicate the potential of these compounds to disturb the secretory functions of the ovaries and uterus which can lead to the deregulation of uterine contractions and to the impairment of fertilisation or to difficulties in the maintenance of gestation.