Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8545719 | Environmental Toxicology and Pharmacology | 2018 | 10 Pages |
Abstract
Although neurotoxicity and hepatotoxicity have long been associated with exposure to polychlorinated biphenyls (PCBs), less is known about the selective toxicity of those hydroxylated PCBs (OH-PCBs) and PCB sulfates that are metabolites derived from exposure to PCBs found in indoor air. We have examined the toxicity of OH-PCBs and PCB sulfates derived from PCBs 3, 8, 11, and 52 in two neural cell lines (N27 and SH-SY5Y) and an hepatic cell line (HepG2). With the exception of a similar toxicity seen for N27 cells exposed to either OH-PCB 52 or PCB 52 sulfate, these OH-PCBs were more toxic to all three cell-types than their corresponding PCB or PCB sulfate congeners. Differences in the distribution of individual OH-PCB and PCB sulfate congeners between the cells and media, and the ability of cells to interconvert PCB sulfates and OH-PCBs, were important components of cellular sensitivity to these toxicants.
Keywords
DMEMRPMI mediumPCB 52OH-PCBDPBSHSAPCB3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide4-ChlorobiphenylMTThuman serum albuminPolychlorinated biphenylhorse serumNeurotoxicityHepatotoxicityDulbecco’s phosphate buffered salinelactate dehydrogenaseLDHDulbecco’s modified eagle’s mediumRoswell Park Memorial Institute mediumhydroxylated polychlorinated biphenyl
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Authors
Eric A. Rodriguez, Brigitte C. Vanle, Jonathan A. Doorn, Hans-Joachim Lehmler, Larry W. Robertson, Michael W. Duffel,