Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8545970 | Environmental Toxicology and Pharmacology | 2018 | 31 Pages |
Abstract
Thymoquinone (TQ) is an active ingredient of some medicinal herbs. Despite extensive studies on the biological and pharmacological properties of TQ, its effect on the characteristics of stem cells remains to be clarified. Therefore, this study was aimed to investigate the effect of TQ on viability, proliferation and immunomodulatory potential of mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) in vitro. The BM-MSCs were isolated from young NMRI mice. The cytotoxic effect of TQ on the BM-MSCs was evaluated using MTT assay. Then, the effect of TQ on the proliferation of BM-MSCs and the mRNA expression of genes involved in self-renewal and immunomodulatory potential of MSCs was assessed by the cell counting and real-time PCR assays. Results showed that TQ reduces the number of BM-MSCs in a dose- and time-dependent manner. In addition, the half-maximal inhibitory concentration values of TQ on the BM-MSCs were 8 μg/ml at 24h and 4 μg/ml at 48 and 72h after treatment. Furthermore, about 90% of the BM-MSCs were alive after treatment with concentrations â¤2 μg/ml of TQ for 24h. The results of cell counting assay indicated that TQ at concentrations of 1-2 μg/ml significantly enhanced the proliferation of BM-MSCs (P < 0.05). The gene expression analysis also showed that Tlr3, Tlr4, Ccl2, Ccl3, Sox2, and Rex1 are overexpressed (Fold change â¥1.5) in the TQ-treated BM-MSCs compared with the untreated samples. In conclusion, these findings propose that TQ may regulate self-renewal and immunomodulatory potential of MSCs. However, the exact mechanisms and the roles of this regulation are required to be elucidated in further study.
Keywords
mitogen-activated protein kinase kinase 3Oct4CCLWntSox2CX3CR1TLRp38CX3C chemokine receptor 1qRT-PCRMKK3Rex1LIFSRY (sex determining region Y)-Box 2IC50MSCsGSK3-β3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidec-MycMAPKMTTGene expressionProliferationImmunomodulationThymoquinoneToll-like receptorSelf-renewalMesenchymal stem cellsleukemia inhibitory factoroctamer-binding transcription factor 4chemokine (C-C motif) ligandbone marrowhalf-maximal inhibitory concentrationquantitative real-time PCRmitogen-activated protein kinaseglycogen synthase kinase 3 beta
Related Topics
Life Sciences
Environmental Science
Health, Toxicology and Mutagenesis
Authors
Elham Alimoradi, Sajjad Sisakhtnezhad, Hassan Akrami,