Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8546163 | Environmental Toxicology and Pharmacology | 2016 | 5 Pages |
Abstract
To investigate the adverse effect of dibutyl phthalate (DBP) on Leydig cells and its mechanism related to gap junction, Leydig cells isolated from adult rats were treated with 0.1% dimethylsulfoxide (DMSO), 50 mg/L DBP, 50 mg/L DBP + 10 μM prostaglandin E2 (PGE2) and 40 μM flutamide respectively. Radioimmunoassay, semi-quantitative RT-PCR, immunofluorescence and Western blot were applied to determine the expression of testosterone and Connexin 43 (Cx43) in Leydig cells. The expression of testosterone and Cx43 were both decreased in DBP group (P < 0.05). While Cx43 was up-regulated after administered to PGE2, there was no significant change in testosterone. However, testosterone was down-regulated with a significant decrease of Cx43 in flutamide group. The results indicated that the inhibitory effect of DBP on testosterone production was not through the down-regulation of Cx43. On the contrary, the change of testosterone can influence the expression of Cx43 in Leydig cells.
Keywords
PGE2EEDBTB3β-HSDGJICHBSSdibutyl phthalateRT-PCRS.E.MDBPFBSCX43DMSOgap junctional intercellular communicationsodium dodecyl sulfate-polyacrylamide gel electrophoresisSDS-PAGEanalysis of varianceANOVAtestosteronestandard error of the meanDibutyl phthalate (DBP)DimethylsulfoxideStarfetal bovine serumLeydig cellgap junctionBlood-testis barrierHank’s balanced salt solutionreverse transcription polymerase chain reactionSteroidogenic acute regulatory proteinProstaglandin E2connexin 43Androgen Receptor
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Authors
Jing Zhang, Shuguang Jin, Jinchang Zhao, Huan Li,