Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8551243 | Regulatory Toxicology and Pharmacology | 2018 | 9 Pages |
Abstract
This article aims to conduct toxicity test research on honokiol microemulsion(HM) to provide reference frame for the safe dose design as well as the toxic and adverse reaction monitoring in clinic. High performance liquid chromatography (HPLC) method was adopted to determine the concentration, stability and uniformity of HM and the results indicated that the test sample was conformed to the toxicity test requirements. In the acute toxicity test, six intravenous drip dosages, namely, 100.0, 66.7, 44.4, 19.8, 8.8, and 3.9â¯mg/kg were set, with one beagle dog in each dosage, respectively. In addition, the results also demonstrated that the approximate lethal dose range of HM was 66.7-100.0â¯mg/kg. In the subchronic toxicity test, beagle dogs were intravenously dripped with HM at doses of 1.25, 0.25 and 0.05â¯mg/kg for 30 days. During the test period, signs of gross toxicity, behavioral changes, body weight, rectal temperature, food consumption, ophthalmoscopy, electrocardiography, urinalysis, blood biochemistry, coagulation, hematology, organ weights and histopathology were examined. Under the present study conditions, the no-observed-adverse-effect level for HM was estimated to be 0.25â¯mg/kg. According to the results of bacterial reverse mutation, chromosomal aberration and micronucleus assays, HM exhibited no notable genotoxicity both in vivo and in vitro.
Keywords
GGTCRERSDMMCDICBLDFIBAPTTPCEALBWBCHCTChlRDWRBCALT4-NQOLEUNCEMCHKETPLTPDWMCHCMPVMCVTCHOMitomycin CPCTTBILNaN3reticulocyteurobilinogen2-AF2-aminofluorene4-nitroquinoline-N-oxideA/GASTAspartate aminotransferaseAlanine aminotransferaseAlbuminalbumin/globulinALPAlkaline phosphatasepolychromatic erythrocytesnormochromatic erythrocytesUric acidrelative standard deviationbilBilirubinanalysis of varianceANOVAtriglycerideMean corpuscular volumeBUNOccult bloodactivated partial thromboplastin timethrombin timeprothrombin timesodium azideAcute toxicitySubchronic toxicityGenotoxicityBeagle dogCyclophosphamideNITretiRed cell distribution widthPlatelet distribution widthFibrinogenlactate dehydrogenaseLDHLymphocyteLeucocytemean platelet volumemean corpuscular hemoglobinMonocyteMean corpuscular hemoglobin concentrationPROurea nitrogenNitritehematocritHemoglobinHgbSpecific gravityProteinPlateletPolyploidyKetoneCreatine kinasecreatininehigh performance liquid chromatographyHPLCRing chromosomeDicentric chromosometotal bilirubintotal proteintotal cholesterolGamma-glutamyl transferasewhite blood cellred blood cellGluGlucose
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Authors
Xiujun Qin, Jingjing Yin, Wei Zhang, Jianguo Li, Jianhua Wen, Shizhong Chen,