Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8552058 | Reproductive Toxicology | 2018 | 34 Pages |
Abstract
Peroxisome proliferator-activated receptor (PPAR-γ) is a nuclear receptor that is highly expressed in placenta. In this study, the PPAR-γ regulated gene networks were characterized in human primary trophoblast cells in vitro. The trophoblasts were isolated from full term placenta after delivery and exposed to 20 μM of the PPAR-γ agonist, pioglitazone, for 72âh and gene expression profiles were examined. Differential expression of selected genes was confirmed with RT-qPCR. Ingenuity pathway analysis was performed to identify PPAR-γ induced biological functions and downstream signaling pathways. In response to pioglitazone treatment, 37 genes were upregulated and 42 genes were downregulated as compared to control cells. The upregulated genes included those involved in metabolic pathways, whereas the expressions of many cytokines and chemokines were downregulated. Our data indicate that PPAR-γ possesses pleiotropic functions also in term trophoblasts regulating genes especially involved in cellular growth and proliferation, inflammatory and immunomodulatory responses and lipid metabolism.
Keywords
BNFHMOX1STAT3CXCLCCLMUC1IPAheme-oxygenase-1C-C motif chemokine ligandPPARSyncytiotrophoblastLRP5GEOS100A8CyPPAHElovl4Extravillous cytotrophoblastTNFMMPScTVLDLRHCGinterleukinbeta-naphthoflavoneIngenuity Pathway AnalysisPathway analysisTrophoblasttumor necrosis factor αCytotrophoblastVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)matrix metalloproteinasesignal transducer and activator of transcription 3gene expression profilingmucin-1Polyaromatic hydrocarbonLow-density lipoprotein receptor-related protein 5Gene Expression OmnibusPioglitazonehuman chorionic gonadotropinperoxisome proliferator-activated receptorVery low-density lipoprotein receptor
Related Topics
Life Sciences
Environmental Science
Health, Toxicology and Mutagenesis
Authors
Rami El Dairi, Pasi Huuskonen, Markku Pasanen, Jaana Rysä,