Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8552379 | Reproductive Toxicology | 2018 | 37 Pages |
Abstract
Leydig cells are the major testosterone-producing cells of the male reproductive system, and damage to these cells can impair fertility of men. Bisphenol A (BPA) is one of the chemicals with the highest volume of production worldwide. The aim of this study was to evaluate the effects of BPA on the growth, viability, and testosterone production of TM3 murine Leydig cells after exposure to BPA for 24 or 48â¯h. BPA reduced testosterone production, cell viability and cell growth in a concentration-dependent manner. The highest tested concentration of BPA (100â¯Î¼M) increased cellular death, as indicated by an increased sub-G1 phase population and a larger number of cells labeled with Hoechst 3342. This concentration of BPA also decreased the number of metabolically active mitochondria as revealed by rhodamine staining. Therefore, our data show that BPA is toxic to Leydig TM3 cells and impairs their steroidogenic function.
Keywords
BPAcytochrome P450 family 11 subfamily A member 1RTCACYP11A1ERβERαIC503-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideDMSOMTTBisphenol AtestosteroneDimethyl sulfoxideStarLeydig cellCell deathhalf-maximal inhibitory concentrationluteinizing hormoneSteroidogenic acute regulatory proteinAndrogen ReceptorEstrogen receptorEstrogen receptor alphaEstrogen receptor beta
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Authors
Gessica Dutra Gonçalves, Simone Cristine Semprebon, Bruna Isabela Biazi, Mario Sergio Mantovani, Glaura Scantamburlo Alves Fernandes,