Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8553851 | Toxicology in Vitro | 2018 | 33 Pages |
Abstract
The use of iron oxide nanoparticles for different biomedical applications, hold immense promise to develop negative tissue contrast in magnetic resonance imaging (MRI). Previously, we have optimized the labelling of mesenchymal stem cells (MSCs) with iron oxide nanoparticles complexed to different transfection agents like poly-l-lysine (IO-PLL) and protamine sulfate (Fe-Pro) on the basis of relaxation behaviour and its biological expressions. However, there is a distinct need to investigate the biocompatibility and biosafety concerns coupled with its cytotoxicity and genotoxicity. This study was prepared to evaluate the viability of cells, generation of ROS, changes in actin cytoskeleton, investigation of cell death, level of GSH and TAC, activities of SOD and GPx, and stability of DNA in MSCs after labelling. Results demonstrated a marginal alteration in toxicological parameters like ROS generation, cell length, actin cytoskeleton, total apoptosis and DNA damage was detected after stem cell labelling. Insignificant depletion of GSH and SOD level, and increase in GPx and TAC level in MSCs were measured after labelling with IO-PLL and Fe-Pro complexes, which later on recovered and normalized to its baseline. This MSCs labelling could provide a reference guideline for toxicological analysis and relaxometry based in vivo MRI detection.
Keywords
Ultrasmall superparamagnetic iron oxideMMSCsGPXGSHPBSSPIOFBSTACPLLMSCsICCSCGEUSPIOROSAntioxidant enzymeSingle-cell gel electrophoresisMRISuperparamagnetic iron oxideImmunocytochemistryanalysis of varianceANOVAMagnetic resonance imagingSODfoetal bovine serumMesenchymal stem cellsGenotoxicitySuperoxide dismutaseTotal antioxidant capacityphosphate buffer salinePoly-l-lysinereduced glutathioneGlutathione peroxidiseReactive oxygen species
Related Topics
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Environmental Science
Health, Toxicology and Mutagenesis
Authors
Sushanta Kumar Mishra, Subash Khushu, Gurudutta Gangenahalli,