Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8554171 | Toxicology in Vitro | 2017 | 40 Pages |
Abstract
Here, we have selected an ITS (Integrated Testing Strategy) for skin sensitization which focuses on three in vitro methods that covered the first three steps of the AOP (DPRA, SENS-IS or h-CLAT). The aim of this study was to compare these three methods due to the WoE approach based on a 2-out-of-3-assessment. The results of 33 references were compared to in vivo data (especially human). We have shown that tested firstly DPRA and SENS-IS have permitted to conclude on 29 of 33 chemicals, whereas DPRA and h-CLAT on 25, and SENS-IS and h-CLAT on 23. With this sequence, DPRA and SENS-IS and then h-CLAT in case of equivocal results, we conclude more quickly by performing fewer tests. Thereby, we have shown that it is better to follow a preferential sequence than testing chemicals simultaneously with these three methods.
Keywords
h-CLATRT-PCRGPTSDNCBACDTNBSMIEDPRAECVAMMUSSTPBSLLNAQRARFIWOEOECD TGHRIPTSN2Acylating agentsFACSAOPMFIITSTMRSLSRHESTS2,4,6-trinitrobenzenesulfonic acidDMSOROShuman cell line activation testLocal lymph node assayDirect Peptide Reactivity AssayQuantitative risk assessmentIntegrated testing strategyReconstructed human epidermisimmunoglobulin QSARHuman Repeated Insult Patch TestSkin sensitizationGuinea pigAllergic contact dermatitisfluorescence-activated cell sortingTest guidelinesDinitrochlorobenzeneDimethyl sulfoxideQuantitative structure-activity relationshipIntegrated approaches to testing and assessmentmolecular initiating eventKey eventsodium lauryl sulfateDendritic cellstimulation indexrelative fluorescence intensityantioxidant response elementtrue positivefalse positivePhosphate-buffered salineEuropean Centre for the Validation of Alternative Methodsadverse outcome pathwayfalse negativetrue negativemean fluorescence intensityAREreverse transcription-polymerase chain reactionWeight of evidenceMichael acceptorsReactive oxygen speciesIATA
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Authors
Elodie Clouet, Saadia Kerdine-Römer, Pierre-Jacques Ferret,