Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8554176 | Toxicology in Vitro | 2017 | 41 Pages |
Abstract
The use of electronic cigarette (e-cig) can be considered as an alternative to smoking. However, due to a lack of thorough toxicological studies, absolute safety of these products cannot be guaranteed. The aim of this in vitro work was to investigate the potential toxicity of e-vapors generated by a smoking machine in human bronchial epithelial BEAS-2B cells cultured at air-liquid interface, in comparison to cigarette smoke (CS). Although CS decreased strongly cell viability from 48Â min exposure, e-vapors induced no cytotoxicity up to 288Â min exposure. Moreover, oxidative stress was evidenced only after exposure to CS, with a decrease secretion of GRO-É from 8Â min and of IL-8 and MCP-1 after 48Â min exposure. Only a low increase of IL-6 secretion was measured in cells exposed to e-vapors. Finally, transcriptomic data of exposed cells indicated that a large number of genes were deregulated in response to CS, especially genes involved in important biological functions as oxidative stress and cell death, while e-vapors elicited very discrete modulation. These results strongly suggest a lower toxicity of e-vapors compared to CS in the BEAS-2B cell line and constitute a baseline for further experimental studies with a larger spectrum of e-liquids and e-cig models.
Keywords
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Health, Toxicology and Mutagenesis
Authors
Sébastien Anthérieu, Anne Garat, Nicolas Beauval, Mélissa Soyez, Delphine Allorge, Guillaume Garçon, Jean-Marc Lo-Guidice,