Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
855891 | Procedia Engineering | 2015 | 4 Pages |
Abstract
We present a method to supply oxygen to on-chip stored aqueous droplets surrounded by oxygen-permeable fluorinated oil in micro-channels. By generating air-droplets (air-bubbles) in between the aqueous droplets, oxygen diffuses to those aqueous droplets. This is confirmed by experiments comparing the effect of nitrogen bubbles and air-bubbles. The proposed method and device are a step towards long-term on-chip cultivation of mammalian cells and aerobic bacteria growth in droplets.
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