Optical Monitoring of Therapeutic Drugs with a Novel Fluorescence- Based POCT Device

A novel optical biochip for immunosuppressants detection in transplanted patients is described. The optical working of the chip, based on total internal reflection fluorescence and consisting of two bonded polymeric parts, a Zeonex 330 slide and a Topas foil was demonstrated. In parallel, a heterogeneous competitive immunoassay for tacrolimus was implemented using a thirteen- microchannel optical chip interrogated by an in-house optical platform based on fluorescence anisotropy and the bioassay conditions were optimized: a limit of detection of 0.11 ng/mL, a limit of quantification of 0.57 ng/mL and a coefficient of variation of 25% were achieved.