Characterization of Interfacial Hydrodynamics in a Single Cell of Shaken Microtiter Plate Bioreactors Applying Computational Fluid Dynamics Technique

Development of orbital shaking technology for enhanced mixing with lower mechanical demand has been receiving significant attention since the advent of advanced mixing schemes. Amongst shaken bioreactors,microtiter plates play significant role both for research and industrial purposes due to their capability of handling tiny amount of liquid in parallel experimentations. Detailed understanding of complicated Flow hydrodynamics thus seems to be considered a continual effort, as it is responsible for efficient gas-liquid mass transfer. Computational fluid dynamics (CFD) technique is shown to be a suitable numerical method in particular for discovering concealed flow facts, which can reliably predict the unsteady state flow within individual plate cells. In this study, CFD is employed to account for gas-liquid interface shape determination to reflect details of hydrodynamics for a novel geometrically modified microtiterplate, which would address diverse unknown flow characteristics vital for accurate mass transfer calculations.