Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8626184 | International Journal of Developmental Neuroscience | 2016 | 21 Pages |
Abstract
GABA treatment reduced total signals in unstimulated networks and networks stimulated for 5Â days, but instead increased signaling in networks stimulated for 1Â day. This increase was prevented by co-treatment with (2R)-amino-5-phosphonopentanoate and 6-cyano-7-nitroquinoxaline-2,3-dione, confirming that GABA invoked excitatory activity in networks stimulated for 1Â day. Glutamate increased signals in networks subjected to all stimulation regimens; the GABA receptor antagonist bicuculline prevented this increase only in networks stimulated for 1Â day. These latter findings are consistent with the induction of so-called “mixed” synapses (which release a combination of excitatory and inhibitory neurotransmitters) in networks stimulated for 1Â day, and support the hypothesis that a critical level of excitatory activity fosters the developmental transition of GABAergic neurons from excitatory to inhibitory.
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Authors
Mikaela Therrien, Rishel Vohnoutka, Edward Boumil, Mary Guaraldi, Sangmook Lee, Thomas B. Shea,