Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8626350 | Placenta | 2018 | 11 Pages |
Abstract
The placenta sheds extracellular vesicles (EVs), including exosomes, into the maternal circulation. We recently demonstrated that this trafficking of EVs is biâdirectional; with uptake of macrophage exosomes by the placenta inducing cytokine release. The specificity of this response is currently unknown. THP-1â¯cells were cultured as monocytes or differentiated to macrophages, and EVs isolated by ultra-centrifugation. The effect of EVs on human placental explants was measured by cytokine ELISA/luminex. Macrophage, but not monocyte, EVs induce the release of pro-inflammatory cytokines by the placenta. Thus, placental responses to immune cell EVs, including exosomes, reflects the phenotype of the source cell.
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Authors
Thomas F. Rice, Beverly Donaldson, Marielle Bouqueau, Beate Kampmann, Beth Holder,