| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8628986 | Cytokine | 2018 | 11 Pages |
Abstract
Intra-assay variance of each mediator was <10%. Inter-assay variance ranged between 1.6 and 37% with an average of 12.2%. Recoveries were similar for all mediators (mean 99.8 ± 2.6%) with a range between 89-107%. Next we measured all mediators in serum, EDTA plasma and sodium heparin plasma of 43 healthy control donors. Of these markers only 19 showed similar expression profiles in the 3 different matrixes. Only 5 mediators were effected by multiple freeze-thawing cycles. Principal component analysis revealed different coagulants cluster separately and that sodium heparin shows the most consistent profile.
Keywords
SLPILAPSCFCXCIL-1RPAI-1OPNHPETPORBP4OPGHGFC5aPlGFDKK1TIMP-1TWEAKsCD163TACITSLPLAIR-1sCD14sCD40LFas-Lfibroblast growth factor-basicLIFTNFfatty acid binding protein-4serum amyloid A1complement 5aMMPDPP-IVCTGFACEFABP4hs-CRPNGFFASOSMPD-1PDGF-BBEGFBDNFAngiotensin-converting enzymeAPRILOsteoprotegerinOsteopontinValidationoncostatin MinterferonIFNinterleukintissue inhibitor of metalloproteinases 1Thrombospondin-1ThrombopoietinTie-2dipeptidyl peptidase IVSerumCytokineCystatin CMIFepidermal growth factorplacenta growth factorHepatocyte growth factorleukemia inhibitory factorMacrophage migration inhibitory factorgranulocyte-macrophage colony-stimulating factorgranulocyte colony-stimulating factorVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)Connective tissue growth factornerve growth factorStem Cell FactorG-CSFBrain-derived neurotrophic factortumor necrosis factorThymic stromal lymphopoietinLuminexmatrix metalloproteinasemacrophage colony-stimulating factorProgrammed death 1plasminogen activator inhibitor 1secretory leukocyte protease inhibitorKidney injury molecule-1Intercellular adhesion molecule 1vascular cell adhesion molecule-1lightAnticoagulantshigh sensitive C-reactive proteinRetinol binding protein 4PlasmaIL-1 receptorTriggering Receptor Expressed on Myeloid cells 1
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Endocrinology
Authors
Rianne C. Scholman, Barbara Giovannone, Sanne Hiddingh, Jenny M. Meerding, Beatriz Malvar Fernandez, Mariska E.A. van Dijk, Mariëlle J. Tempelman, Berent J. Prakken, Wilco de Jager,