Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8630763 | General and Comparative Endocrinology | 2018 | 41 Pages |
Abstract
As the close paralog of adiponectin, C1q/TNF-Related Protein 9 (CTRP9) has been reported to be involved in the regulation of glucose and fat metabolism, immunization and endothelial cell functions. However, information regarding the actions of Ctrp9 on reproduction is extremely limited in fish. As a first step, Ctrp9, adiponectin receptor 1 (Adipor1) and Adipor2 were identified from Nile tilapia. The open reading frame (ORF) of ctrp9 was 1020â¯bp which encoded a 339 amino acids. Moreover, the ORFs of adipor1 and adipor2 were 1131â¯bp and 1134â¯bp encoding 376 and 377 amino acids, respectively. Tissue distribution showed that ctrp9 mRNA levels were highest in the kidney in both sexes. And, the expression of adipor1 and adipor2 were widely distributed in all tissues examined, exhibiting high levels in the brain, gonad, gut and stomach. In addition, intraperitoneal (i.p.) injection of gCtrp9 (globular Ctrp9) suppressed the hypothalamic expression of gnrh2 (gonadotropin-releasing hormone 2) and gnrh3, as well as gthα (gonadotropic hormone α), fshβ (follicle-stimulating hormone β), lhβ (luteinizing hormone β), lhr (LH receptor) and fshr (FSH receptor) mRNA levels in the pituitary. The mRNA levels of adipor1, but not adipor2, in the gonads were also inhibited after injection. Moreover, the levels of serum E2 (estrogen) in female and T (testosterone) in male were significantly decreased after injection of gCtrp9. Overall, our data provides novel data indicating, for the first time, a regulatory effect of CTRP9 on teleost reproduction.
Keywords
AMPKHPGi.c.v.GPCRERK 1/2eNOSPPARαAdipoRHMWi.p.POMCNPYCTRP9PAQRORFTNFαG protein-coupled receptorsperoxisome proliferator-activated receptor alphaenzyme-linked immuno sorbent assayEstradiol-17βHCGKISSELISAtestosteroneReproductiontumor necrosis factor αTilapiaintraperitonealIntraventricularCNSendothelial nitric oxide synthasecentral nervous systemopen reading frameGonadotropin-releasing hormonehigh molecular weightproopiomelanocortinKisspeptinextracellular signal-regulated kinaseAMP-activated kinasehuman chorionic gonadotropinadiponectin receptorFSH receptorLH receptorNeuropeptide Y
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Authors
Guokun Yang, Qinqin Song, Caiyun Sun, Jingkai Qin, Jirong Jia, Xi Yuan, Yazhou Zhang, Wensheng Li,