Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8633241 | Neuropeptides | 2018 | 44 Pages |
Abstract
The central administration of glucagon-like peptide-2 (GLP-2) decreases blood pressure in rats. In the present study, we investigated the hypotensive effects of GLP-2 using spontaneously hypertensive rats (SHRs), an animal model of hypertension. The central administration of GLP-2 (0.6â¯Î¼g) decreased mean arterial pressure (MAP) in SHRs (â24.1â¯Â±â¯4.5%; Pâ¯<â¯0.05), but not in normotensive Wistar-Kyoto (WKY) rats (â10.6â¯Â±â¯7.4%; Pâ¯>â¯0.05), whereas GLP-2 (6â¯Î¼g) decreased MAP in WKY rats (â23.5â¯Â±â¯4.2%; Pâ¯<â¯0.05) and SHRs (â46.7â¯Â±â¯11.6%; Pâ¯<â¯0.01) under anesthesia with urethane and α-chloralose. Histological analyses revealed that the central administration of GLP-2 (6â¯Î¼g) induced Fos immunoreactivity (Fos-IR) in the hypothalamic and medullary areas in WKY rats and SHRs. However, the distribution of Fos-IR in GABAergic neurons in the rostral ventrolateral medulla (RVLM) differed between WKY rats and SHRs. GLP-2 directly modulated the excitability of RVLM neurons in brainstem slices from SHRs, but not WKY rats. These results suggest that neuronal activity through the activation of GLP-2 receptors in the RVLM contributes to lowering blood pressure in SHRs.
Keywords
DABSHRSPGLP-2RWKYVMHPVHGLP-2SHRspro-opiomelanocortinAMBCVLMRVLMNTSDMHintracerebroventricularGADICVPOMCglutamic acid decarboxylaserostral ventrolateral medullaHypotensionFos immunoreactivityImmunohistochemistrytyrosine hydroxylaseCNSdiaminobenzidinecentral nervous systemwhole-cell recordingsarea postremamean arterial blood pressureFos-irlocus coeruleuscaudal ventrolateral medullaSpontaneously hypertensive ratsmapnucleus of the solitary tractNucleus ambiguousdorsomedial hypothalamic nucleusVentromedial hypothalamic nucleusparaventricular nucleus of the hypothalamusWistar-Kyotoc-fos proteinGlucagon-like peptide-2
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Authors
Sachie Sasaki-Hamada, Koji Narusawa, Ryuji Nakamura, Hitoshi Ishibashi, Jun-Ichiro Oka,