Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8644842 | Gene | 2018 | 9 Pages |
Abstract
HSP90β1 (known as glyco-protein 96, GP96) is a vital endoplasmic reticulum (ER) depended chaperonin among the HSPs (heat shock proteins) family. Furthermore, it always processes and presents antigen of the tumor and keeps balance for the intracellular environment. In the present study, we explored the effect of tributyltin chloride (TBT-Cl) exposure on HSP90β1 expression in river pufferfish, Takifugu obscurus. The full length of To-HSP90β1 was gained with 2775â¯bp in length, with an ORF (open reading frame) encoding an 803 aa polypeptide. A phylogenetic tree was constructed and showed the close relationship to other fish species. The HSP90β1 mRNA transcript was expressed in all tissues investigated with higher level in the gill and liver. After the acute and chronic exposure of TBT-Cl, the To-HSP90β1 mRNA transcript significantly was up-regulated in gills. Moreover, the histology study indicated the different injury degree of TBT-Cl in liver and gill. Immunohistochemistry (IHC) staining results implied the cytoplasm reorganization after TBT-Cl stress and the function of immunoregulation for To-HSP90β1 to TBT-Cl exposure. All the results indicated that HSP90β1 may be involved in the resistance to the invasion of TBT-Cl for keeping autoimmune homeostasis.
Keywords
mRNAH & Egp96ORFTributyltin chlorideNCBIkDaGRPImmunologic functionhistocompatibility complexUPRHSPsSPSIgGqPCRLC50PBSMS-222BiPcDNAComplementary DNAAdenosine TriphosphateATPamino acidBasic Local Alignment Search Toolstandard deviationBlastImmunohistochemistryIHCimmunoglobulin Gtricaine methanesulfonatebase pairmessenger ribonucleic acidendoplasmic reticulumopen reading frameMHCPhosphate-buffered salineNational Center for Biotechnology InformationUTR یا untranslated regions untranslated regionIsoelectric pointhematoxylin-eosinquantitative real-time PCRUnfolded protein responseStress proteinsglucose-regulated proteinHeat shock proteinskilo-dalton
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Authors
Xu Dong-po, Fang Di-an, Zhao Chang-sheng, Jiang Shu-lun, Hu Hao-yuan,