Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8644880 | Gene | 2018 | 7 Pages |
Abstract
As a well-conserved microRNA, miR-183 is ubiquitously expressed in many tissues and cells including backfat and the 3T3-L1 adipocytes; however, the mechanisms regulating miR-183 in adipogenesis remain poorly understood. Here, we explored the expression pattern and role of miR-183 in adipogenesis using hircine preadipocytes. The results showed that miR-183 was up-regulated during preadipocyte differentiation, and overexpression of miR-183 enhanced lipid accumulation and dramatically increased the mRNA expression of the adipogenic genes PPARγ, C/EBPα, SREBP-1c, FAS, and ACC. Using bioinformatics tools, we predicted Smad4 to be a target of miR-183. This was subsequently validated with a luciferase reporter assay. Overexpression of miR-183 suppressed the mRNA and protein levels of Smad4 significantly, whereas inhibiting miR-183 had the opposite effect. However, inhibition of Smad4 greatly accelerated lipid deposition and increased the expression of adipogenic genes which consists with the results of miR-183 overexpression. In conclusion, these results indicate that miR-183 promotes hircine preadipocyte differentiation by targeting Smad4.
Keywords
ACCSREBP-1cFASTGFβPPARγLRP6SMAD4PDK1miR-183C/EBPαc/ebpβC/EBPδCCAAT/enhancer binding protein αAdipogenesisacetyl-CoA carboxylasefatty acid synthaseGoatTransforming growth factor βPhosphoinositide-dependent kinase-1PreadipocytesCCAAT/enhancer binding protein βSterol regulatory element binding protein 1cperoxisome proliferator-activated receptor γ
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Genetics
Authors
Wei Zhao, Hailong Yang, Juntao Li, Yuan Chen, Jiaxue Cao, Tao Zhong, Linjie Wang, Jiazhong Guo, Li Li, Hongping Zhang,