Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8644910 | Gene | 2018 | 9 Pages |
Abstract
Aldose reductase (AR) is a rate-limiting enzyme in the polyol pathway and is also the key enzyme involved in diabetic complications. The silkworm purple quail-like mutant (q-lp) exhibits pigmented dots on its epidermis. The q-lp mutant also shows developmental abnormalities and decreased vitality. In this study, fat bodies from the q-lp mutant and the wildtype 932VR strain were subjected to two-dimensional gel electrophoresis (2-DE) analysis, and the Bombyx mori AR (BmAR) protein was found to be significantly downregulated in the q-lp mutant. The expression of BmAR at the mRNA level was also significantly downregulated, as verified through quantitative reverse transcription PCR (qRT-PCR). Knockdown of the expression of BmAR via RNAi resulted in a reduction of silkworm weight. The sorbitol level in q-lp was significantly lower than in the wildtype. These results suggested that the BmAR gene is closely related to the development of the q-lp mutant. Investigation of the cause of BmAR downregulation in the q-lp mutant could contribute to revealing the function of AR in insects and offers a new method of identifying AR inhibitors for the treatment of diabetic complications.
Keywords
dsRNAG6PDUGTsNCBIeGFPRPKMNADPH2-DEGAPDHUDP-glycosyltransferasesqRT-PCRRNA interferencedouble-stranded RNARNAiAldose reductaseBasic Local Alignment Search Tooltwo-dimensional gel electrophoresisSDS-PAGESodium dodecyl sulfate polyacrylamide gel electrophoresisBlastreads per kb per million readsquantitative reverse transcription PCRSorbitoldiabetic complicationsNational Center of Biotechnology Informationnicotinamide adenine dinucleotide phosphateGene ontologyenhanced green fluorescence proteinBombyx moriGlucose-6-phosphate isomeraseglucose-6-phosphate dehydrogenaseglyceraldehyde-3-phosphate dehydrogenase
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Authors
Pingyang Wang, Simin Bi, Weiyang Wei, Zhiyong Qiu, Dingguo Xia, Xingjia Shen, Qiaoling Zhao,