Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8644940 | Gene | 2018 | 10 Pages |
Abstract
Genetic mutations in dozens of monogenic genes can lead to serious podocyte dysfunction, which is a major cause of steroid-resistant nephrotic syndrome (SRNS). The NUP160 gene is expressed in both human kidney and mouse kidney. However, whether knockdown of NUP160 impairs podocytes has not yet been established. Therefore, we knocked down NUP160 by targeted short hairpin RNA (shRNA) in conditionally immortalized mouse podocytes and observed the effect of NUP160 knockdown on the proliferation, apoptosis, autophagy and cell migration of podocytes. We also investigated the effect of NUP160 knockdown on the expression and localization of podocyte associated molecules, such as nephrin, podocin, CD2AP and α-actinin-4. The knockdown of NUP160 significantly inhibited the proliferation of podocytes by decreasing the expression of both cyclin D1 and CDK4, increasing the expression of p27, and inducing S phase arrest. The knockdown of NUP160 promoted the apoptosis and autophagy of podocytes, and enhanced cell migration. The knockdown of NUP160 decreased the expression of nephrin, podocin and CD2AP in podocytes, and increased the expression of α-actinin-4. The knockdown of NUP160 also altered the subcellular localization of nephrin, podocin and CD2AP in podocytes. These results suggest that the knockdown of NUP160 impairs mouse podocytes, i.e. inhibiting cell proliferation, inducing apoptosis, autophagy and cell migration of mouse podocytes, and altering the expression and localization of podocyte associated molecules, including nephrin, podocin, CD2AP and α-actinin-4.
Keywords
CCK-8FSGSPARPIFN-γqRT-PCRHRPshRNALSDFBSSRNSSSNSshort hairpin RNAinterferon-γleast significant differencefetal bovine serumSteroid-sensitive nephrotic syndromeSteroid-resistant nephrotic syndromecell counting kit-8Nucleoporinquantitative real-time polymerase chain reactionHorseradish peroxidasePropidium iodidePodocyteoptical densityFocal segmental glomerulosclerosis
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Genetics
Authors
Ping Wang, Feng Zhao, Xiaojing Nie, Jiewei Liu, Zihua Yu,