Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8645098 | Gene | 2018 | 43 Pages |
Abstract
PIN1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that controls cell fate by regulating multiple signal transduction pathways and is found to be overexpressed in a variety of malignant tumors. Herein, we found the expression of PIN1 is up-regulated while miRNA-370 (miR-370) down-regulated in both esophageal squamous-cell carcinoma (ESCC) tissues and cells. Transfection of miR-370 can significantly decrease PIN1 expression in targeting ESCC cells. Overexpression of miR-370 can induce decreased cell proliferation and cell cycle arrest, as well as increased apoptosis in ESCC cells, while this function can be significantly prevented by co-transfection of PIN1. Further experimental results demonstrated that β-catenin, cyclin D1, and caspase activation might be involved in miR-370/PIN1 induced growth inhibition and apoptosis. Besides, low miR-370 and high PIN1 expression significantly correlated with tumor diameter, poor differentiation, tumor invasion and lymph node metastasis in patients diagnosed with ESCC. In conclusion, downregulation of miR-370 in ESCC is associated with cancer progression and promotes cancer cell proliferation via upregulating PIN1, which might be a potential therapeutic target and adverse prognostic factor in the clinic.
Keywords
DMEMBIADnmt1ESCCDNA methyltransferase 1ATTCqRT-PCRERKSPFDulbecco's modified Eagle's mediumEsophageal adenocarcinomaBaxBioinformatic analysisCell proliferationspecific pathogen-freeEsophageal cancerRunt-related transcription factor 3American Type Culture CollectionUTR یا untranslated regions untranslated regionquantitative real-time polymerase chain reactionWestern blottingCancer progressionPin1extracellular signal-regulated kinase
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Authors
Mingzhi Chen, Yang Xia, Yongfei Tan, Guojun Jiang, Hai Jin, Yijiang Chen,