Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8645117 | Gene | 2018 | 6 Pages |
Abstract
BST-2(tetherin/CD317/HM1.24) has been identified as a cellular antiviral factor that inhibits the release of a wide range of enveloped viruses from infected cells. Orthologs of BST-2 have been identified in several species including humans, monkeys, cows, sheep, pigs, and mice. In this study, we cloned the gene and characterized the protein of the BST-2 homolog from sika deer (Cervus nippon). cnBST-2 shares 37.8% and 74.2% identity with the BST-2 homologs from Homo sapiens and Ovis aries, respectively. The extracellular domain of cnBST-2 has two putative N-linked glycosylation sites and three potential dimerization sites. cnBST-2 was shown to be expressed on the cell surface, like human BST-2. Exogenous expression of cnBST-2 resulted in potent inhibition of HIV-1 particle release in 293T cells; however, this activity resisted antagonism by HIV-1 Vpu. Moreover, cnBST-2 was not able to activate nuclear factor-κB, in contrast to human BST-2. This study is the first report of the isolation and characterization of BST-2 from C. nippon.
Keywords
SIVIAVNF-κBVSVBCIPGPiAPOBEC3GPAGEDAPINBTBST-2TRIM5αKSHVSAMHD1eGFPTGNPBMCBLVFBSSDSPBS4′,6-diamidino-2-phenylindole5-bromo-4-chloro-3-indolylphosphateIFNsFIVpolyacrylamide gel electrophoresisinterferonsNitro blue tetrazolium chloridesodium dodecyl sulfatefetal bovine serumPeripheral blood mononuclear cellAntiviralphosphate buffered solutionNitrocelluloseHBVVesicular stomatitis virusInfluenza A virusHIV-1Human Immunodeficiency Virus type-1Bovine leukemia virushepatitis B virusSimian immunodeficiency virusenhanced green fluorescent proteinCervus nipponglycosyl-phosphatidylinositol
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Authors
Jiawen Wang, Shuai Bian, Meichun Liu, Xin Zhang, Siming Wang, Xueyuan Bai, Daqing Zhao, Yu Zhao,